GST标签是一个26kda大小的蛋白质标签，可用于蛋白质纯化。GST是谷胱甘肽-S-转移酶的简称。使用GST标签进行蛋白质纯化是利用酶对底物的特异性来获得最大纯度。
产品特性：
应用 | Specific binding and purification of GST-tagged proteins |
特异性 | Affinity to GST-tagged proteins |
结合能力 | 10 mg / ml |
Bead Ligand | Protein purification beads with GST-Antibody |
粒径大小 | 40 μm |
pH稳定性 | 3-12 |
推荐流速 | 0.25-1.0 mL/min |
推荐操作压力 | <5bar (72 psi) |
保存温度 | 2℃到8℃ |
Cube Biotech公司的GST/Gluthathione亲和琼脂糖已成功发表在以下出版物：
PureCube GLUTHIONE磁珠的效率
图1：可靠的批次间再现性。谷胱甘肽-S-转移酶从大肠杆菌裂解物中纯化，利用三个独立生产不同批次的PureCube GLUTHIONE磁珠。FT：穿流，E1-E5：洗脱组分。
我们的GST标签纯化产品能够复制所有的纯化试验从批次到批次。产品在多次使用后实现了最小的波动，并且多次连续的GST标签纯化在使用相同的磁珠的情况下能确保相同的高质量。
订货信息：
32101 | PureCube Glutathione Agarose (1 ml) |
32103 | PureCube Glutathione Agarose (10 ml) |
32105 | PureCube Glutathione Agarose (50 ml) |
32110 | PureCube Glutathione Agarose (250 ml) |
32112 | PureCube Glutathione Agarose (500 ml) |
32201 | PureCube Glutathione MagBeads（1ml） |
32205 | PureCube Glutathione MagBeads(5ml) |
32225 | PureCube Glutathione MagBeads(25ml) |
32290 | PureCube Glutathione MagBeads(4x25ml) |
32293 | PureCube Glutathione MagBeads(250ml) |
References:
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2. Napolitano, Anna et al. “Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection.” Journal of immunology (Baltimore, Md. : 1950) vol. 201,2 (2018): 604-614. doi:10.4049/jimmunol.1701322
3. Boor, Susanne de (2015). Mechanistic studies on the regulation of Ran-function by post-translational lysine-acetylation. Dissertation, Universität zu Köln.
4. Fischer K. (2015). TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Dissertation, Universität zu Mainz.
5. A. Sommerkamp, et al. (2019). Crystal structure of the first eukaryotic bilin reductase Gt PEBB reveals a flipped binding mode of dihydrobiliverdin. Journal of Biological Chemistry. jbc.RA119.009306. 10.1074/jbc.RA119.009306.
6. Chiocchini C., Trefzer A., Vattem K., Kuhn P., inventor; Thermo Fisher Scientific Geneart GmBH, Pierce Biotechnology Inc, assignee. Devices and methods for producing nucleic acids and proteins. United States patent US20180291413A1. 2016 April 10.
7. Chiocchini, C. et al. (2020). From Electronic Sequence to Purified Protein Using Automated Gene Synthesis and In Vitro Transcription/Translation. ACS Synthetic Biology. XXXX. 10.1021/acssynbio.0c00060.